Mathematics of Cryopreservation: from tissue preparation to freezing and ice formation
Monday, June 14 at 5:45pm (PDT)Tuesday, June 15 at 01:45am (BST)Tuesday, June 15 09:45am (KST)
Robyn Shuttleworth (University of Saskatchewan, Canada), James Benson (University of Saskatchewan, Canada)
Cryopreservation is the process of freezing and storing cells or tissues at very low temperatures. Successful cryopreservation requires several challenging and interdependent steps, where failure in one step often results in complete destruction of the material. Some of these challenges include osmotic effects such as dehydration, solution toxicity damage, ice crystal formation, and thermal stress fractures. Whilst there is often high success with single cell cryopreservation, larger tissue samples prove much more challenging. The cryopreservation of large tissues would enable the prolonged, stable storage of whole organs. This would be beneficial for many reasons, with one of the most compelling being thorough testing and safe delivery of donor organs to their recipients. Because cryopreservation is a series of biophysical and biochemical events, it has a long history of success driven by modeling. Therefore, the aim of this mini symposium is to bring together the mathematical approaches used to analyze the different protocols required for successful cryopreservation. These models will highlight and tackle the challenges we face at each stage of the cryopreservation process and give insight into how we are progressing towards whole organ cryopreservation.